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{"sheet":"Section 1 - Laboratory Info","index":0,"n_rows":14,"n_cols":2,"rows_kept":14,"cut":false,"hidden":false,"merged":[]}
["Hello and welcome to the NORMAN TOP assay questionnaire. The purpose of the work is to better understand the capabilities for TOP Assay analysis across the network and to develop networks and share learning. "]
["To make reviewing the data easier, we have tried to fix responses where appropriate, these cells are shaded green, if suitable please use the dropdown options. Cells are open text is needed. "]
["Please Allow Macros"]
[]
["Section 1 – Laboratory information"]
["Question ","Response"]
["What is the name of your laboratory/organisation"]
["Name of individual filling in the questionnaire? "]
["Contact email address: "]
["Is your laboratory primarily a commercial or research laboratory, or both?"]
["Does your laboratory currently apply TOP Assay analysis?"]
["Is your laboratory an accredited laboratory for PFAS analysis? "]
["How many samples does your organisation analyse per year for PFAS analysis (approximately)?"]
["How many TOP assay samples does your organisation analyse per year for PFAS analysis (approximately)?"]

{"sheet":"Section 2 - TOPA Capabilites","index":1,"n_rows":48,"n_cols":9,"rows_kept":48,"cut":false,"hidden":false,"merged":[[35,0,38,0],[40,0,46,0],[4,0,10,0],[11,0,16,0],[17,0,24,0],[25,0,29,0],[30,0,34,0]]}
["Section 2 – TOPA capabilities  "]
["Please clarify your methods regarding TOPA and on which matrices you are using it now. Feel free to add any missing matrix at the end of the list, in case it's being analysed in your laboratory."]
["Category Matrix","Subcategory Matrix","Do you analyse this media?","Is your method based a method used for other matrices? – if yes, please state which.","Is your method based on a publication or standard method? -If so, please provide a reference.","Have you made any modifications to the method?","Method status","Are you confident with the method?  ","If no, what is causing concern?"]
[null,null,null,"if missing please use text","Preferably a DOI address of method number (open text)","What modifications have you made? (open text) - Details such as, increased reagents, additional extraction step"]
["Water","Water - Drinking Water"]
[null,"Water - Surface (fresh) waters"]
[null,"Water - Surface (saline) waters"]
[null,"Water - Stormwater  "]
[null,"Water - Run-off "]
[null,"Water - Rainwater"]
[null,"Water - Waste Water"]
["Soil and Sediment","Soil and Sediment - Suspended Particulate Matter"]
[null,"Soil and Sediment - Sediment  "]
[null,"Soil and Sediment - Soil"]
[null,"Soil and Sediment - Compost/ mulch"]
[null,"Soil and Sediment - Biosolids/Sewage Sludge / digestate "]
[null,"Dust"]
["Biota (animals)","Biota (animals) - Fish (organ / tissue)"]
[null,"Biota (animals) - Invertebrates"]
[null,"Biota (animals)- Cetacean (organ / tissue)"]
[null,"Biota (animals)- Land Mammals"]
[null,"Biota (animals) - Birds "]
[null,"Biota (animals)- Eggs"]
[null,"Biota (animals)- Milk"]
[null,"Biota (animals)- Blood / serum "]
["Biota (plants and algae) ","Biota (plants and algae)  - Algae"]
[null,"Biota (plants and algae)  - Seaweed"]
[null,"Biota (plants and algae)  - Food (crops)"]
[null,"Biota (plants and algae)  - Whole Plant"]
[null,"Biota (plants and algae)  - Plant parts"]
["Human ","Human  - Whole Blood"]
[null,"Human  - Serum  "]
[null,"Human  - Plasma"]
[null,"Human  - Urine"]
[null,"Human  - Breastmilk"]
["Air and gasses","Air and gasses - Indoor Air "]
[null,"Air and gasses - Outdoor air"]
[null,"Air and gasses - Flue gas  / stack emission "]
[null,"Air and gasses - Biogas "]
["Filter Paper / Cartridges / Passive discs  ","Filter Paper / Cartridges / Passive discs  "]
["Products and Consumables ","Products and Consumables - Paints and Varnishes "]
[null,"Products and Consumables - Cosmetics"]
[null,"Products and Consumables - Paper and cardboard"]
[null,"Products and Consumables - Fabric / carpet"]
[null,"Products and Consumables - Food Packaging (Paper)"]
[null,"Products and Consumables - Food Packaging (other)"]
[null,"Products and Consumables - Rubber and plastic"]
["Other"]

{"sheet":"Section 3 - Method and QA","index":2,"n_rows":51,"n_cols":6,"rows_kept":51,"cut":false,"hidden":false,"merged":[[42,1,44,5]]}
["Section 3 – Method details, Validation, and Quality Assurance (Here the responder will be asked to choose the method/matrix to which the answers apply to).  "]
["This section is designed to capture the key steps executed in the method which may influence the performance and provide information on successful or unsuccessful modifications."]
["The validation questions will provide information on the confidence of the results, reproducibility, and accuracy. This should provide information on good practices within the community."]
["Please fill in a single description per matrix analysed, and copy-paste the format in successive columns to the right, in case more space is needed."]
[null,"Matrix Category 1","Matrix Category 2","Matrix Category 3","Matrix Category 4","Matrix Category 5"]
["Matrix analysed:"]
["Reference for Matrix (if published):"]
["General Approach","Laboratory Response "]
["Analytical Technique (Solid Phase Extraction & LC-MS/MS)"]
["Sample storage"]
["Sample Preparation"]
["Extraction"]
["Sample clean up"]
["Washing Buffer used in clean-up?"]
["Final extract"]
["Advice for others"]
["Oxidation step – one of the key aspects of TOP Assay is the oxidation and base modification of the sample, as a result there is potential for deviation between methods.  "]
["Sample Mass (g or mL please state) "]
["Oxidant used"]
["Concentration of oxidant solution (Molar Concentration)"]
["Volume of oxidant  (mL)"]
["Alkali base used"]
["Concentration of alkali base solution (Molar Concentration)"]
["Volume of alkali base used (mL)"]
["Heating Method "]
["Heating temperature (°C)"]
["Duration of Heating"]
["Additional accelerate / catalyst"]
["Validation, assurance and performance - Please provide details of how quality of analysis was / is assessed "]
["How do you quantify uncertainty"]
["External Reference Material"]
["Internal Reference Material"]
["Laboratory known reference sample (mixed inhouse)"]
["Sample Spiking"]
["Field blank"]
["Procedural blank"]
["Instrumental blank"]
["In blank subtraction performed?"]
["Are precursors analysed following oxidation?"]
["Do you use multiple analytical methods to capture all oxidised PFAS substances?"]
["Do you use labelled or other internal standards? (Please specify)"]
["General Performance of analysis "]
["Typical recovery rates","Please go to Section 3.1 to report the results"]
["Typical analytical precision (%RSD)"]
["Typical recovery when compared with targeted analysis"]
["Data interpretation"]
["No interpretation"]
["Comparison between concentrations before and after oxidation"]
["Comparison of molar concentrations before and after oxidation"]
["Comparison of fluorine masses"]
["Other?"]

{"sheet":"Section 3.1 - Method Performane","index":3,"n_rows":45,"n_cols":21,"rows_kept":45,"cut":false,"hidden":false,"merged":[[3,17,3,20],[3,1,3,4],[4,1,4,4],[3,5,3,8],[3,9,3,12],[3,13,3,16],[4,5,4,8],[4,9,4,12],[4,13,4,16],[4,17,4,20]]}
["Section 3.1 – Typical recovery rates and precision (RSD)"]
["This section is designed to capture recovery rates and precision of the method before and after TOPA"]
["Please, note that here recovery rates are evaluated, therefore, those may be matrix dependant according to your methods described in section 3. Please, add as many copies of the lines as needed in order to show the recovery rates for the different matrixes analysed."]
[null,"Matrix Category 1",null,null,null,"Matrix Category 2",null,null,null,"Matrix Category 3",null,null,null,"Matrix Category 4",null,null,null,"Matrix Category 5"]
["Matrix analysed"]
[null,"Typical recovery for target analysis","Typical analytical precision (%RSD)","Typical recovery for target analysis after TOPA","Typical analytical precision (%RSD)","Typical recovery for target analysis","Typical analytical precision (%RSD)","Typical recovery for target analysis after TOPA","Typical analytical precision (%RSD)","Typical recovery for target analysis","Typical analytical precision (%RSD)","Typical recovery for target analysis after TOPA","Typical analytical precision (%RSD)","Typical recovery for target analysis","Typical analytical precision (%RSD)","Typical recovery for target analysis after TOPA","Typical analytical precision (%RSD)","Typical recovery for target analysis","Typical analytical precision (%RSD)","Typical recovery for target analysis after TOPA","Typical analytical precision (%RSD)"]
[null,"%","%","%","%","%","%","%","%","%","%","%","%","%","%","%","%","%","%","%","%"]
["PFCA - C2"]
["PFCA - C3"]
["PFCA - C4"]
["PFCA - C5"]
["PFCA - C6"]
["PFCA - C7"]
["PFCA - C8"]
["PFCA - C9"]
["PFCA - C10"]
["PFCA - C11"]
["PFCA - C12"]
["PFCA - C13"]
["PFCA - C14"]
["PFCA - C15"]
["PFCA - C16"]
["PFCA - C17"]
["PFCA - C18"]
[]
["PFSA - C2"]
["PFSA - C3"]
["PFSA - C4"]
["PFSA - C5"]
["PFSA - C6"]
["PFSA - C7"]
["PFSA - C8"]
["PFSA - C9"]
["PFSA - C10"]
["PFSA - C11"]
["PFSA - C12"]
[]
["4:2 FTSA"]
["6:2 FTSA"]
["8:2 FTSA"]
["10:2 FTSA"]
["HFPO-DA (GenX)"]
["PFMoPrA"]
["PFECHS"]
["PFEESA"]

{"sheet":"Section 4 - Procedural LOQ","index":4,"n_rows":50,"n_cols":6,"rows_kept":50,"cut":false,"hidden":false,"merged":[[2,0,2,1]]}
["Section 4 – Procedural limits of quantification (pLOQs)"]
["This section is designed to capture semi-quantitively data on limits of quantification."]
["Please, note that here Procedural LOQs are evaluated, therefore, those may be matrix dependant according to your methods described in section 3. Please, add as many copies of the lines as needed in order to show the pLOQs for the different matrixes analysed."]
["pLOQs ranges are noted in ng/L sample. "]
[null,"Matrix Category 1","Matrix Category 2","Matrix Category 3","Matrix Category 4","Matrix Category 5"]
["Matrix analysed"]
["Reference for Matrix (if published):"]
["Sample matrix type (liquid / solid / other)"]
["Brief outline of how LOQ are calculated"]
["Analytical method (eg SPE-WAX, LC-MS/MS)"]
["Significant issues encountered (Yes, No & Brief info)"]
["Units (please use ng/L and indicate if it is solid in the matrix analysed box)","ng/L","ng/L","ng/L","ng/L","ng/L"]
["PFCA - C2"]
["PFCA - C3"]
["PFCA - C4"]
["PFCA - C5"]
["PFCA - C6"]
["PFCA - C7"]
["PFCA - C8"]
["PFCA - C9"]
["PFCA - C10"]
["PFCA - C11"]
["PFCA - C12"]
["PFCA - C13"]
["PFCA - C14"]
["PFCA - C15"]
["PFCA - C16"]
["PFCA - C17"]
["PFCA - C18"]
[]
["PFSA - C2"]
["PFSA - C3"]
["PFSA - C4"]
["PFSA - C5"]
["PFSA - C6"]
["PFSA - C7"]
["PFSA - C8"]
["PFSA - C9"]
["PFSA - C10"]
["PFSA - C11"]
["PFSA - C12"]
[]
["4:2 FTSA"]
["6:2 FTSA"]
["8:2 FTSA"]
["10:2 FTSA"]
["HFPO-DA (GenX)"]
["PFMoPrA"]
["PFECHS"]
["PFEESA"]

{"sheet":"Section 5 - Additional info","index":5,"n_rows":19,"n_cols":2,"rows_kept":19,"cut":false,"hidden":false,"merged":[]}
["Section 5 – Additional information"]
["Please, include some additional explanations on your TOPA calculations, issues, and intentions towards improvement."]
[]
["Question","Response"]
["Do you routinely compare TOPA results with target analysis?"]
["Do you use complementary suspect, or non-target methods?"]
["Have you done any work on short chain C2 and C3 PFAS in TOPA analysis? (if yes please provide short description)"]
["Are there particular groups of PFAS you are having difficulty working with? - Please list "]
[]
["Best practice for calculating mass balance?"]
["Conversion method (how you convert the amounts of known PFCA precursors into amounts of PFCAs upon oxidation)"]
["What resource do you use for molar mass conversions"]
[]
["If you use extractable organofluorine method- which type of extraction method are you using?"]
["If you use adsorbable organofluorine  method- which type of extraction method are you using?"]
["Do you also use the method for non-PFAS substances – other compounds?"]
[]
["Have you participated in any external comparisons of reference standards? E.g. Ring Tests, Proficiency Testing and certified standards. (Please specify)"]
["Are you happy to participate in an interlaboratory assessment of the methods, what analysis in what matrix – using the inhouse methods. This could include the opportunity to use a common extraction methods with the inhouse analysis."]

{"sheet":"DropDown List options ","index":6,"n_rows":46,"n_cols":7,"rows_kept":46,"cut":false,"hidden":true,"merged":[]}
["List Matrix Category","List Matrix Sub-Category","Method Status","Method Confidence ","Method Concern","Typical Recovery rates","Analytical Precision"]
["Water","Water - Drinking Water","Fully Accredited ","High","Blank Contamination","<70%","<10%"]
["Soil and Sediment","Water - Surface (fresh) waters","In House Accredited ","Medium ","Poor Mass balance","70% - 90%","10% - 20%"]
["Biota (animals)","Water - Surface (saline) waters","In Development ","Low ","Poor recovery","90% - 110%","20% - 30%"]
["Biota (plants and algae) ","Water -","Research and Development","I don’t know! ","Incomplete oxidation ","110% - 130%","30% - 40%"]
["Human ","Water - Stormwater  ",null,null,"Matrix issues",">130%",">50%"]
["Air and gasses","Water - Run-off "]
["Filter Paper / Cartridges / Passive discs  ","Water - Rainwater"]
["Products and Consumables ","Water - Waste Water"]
["Other","Soil and Sediment - Suspended Particulate Matter"]
[null,"Soil and Sediment - Sediment  "]
[null,"Soil and Sediment - Soil"]
[null,"Soil and Sediment - Compost/ mulch"]
[null,"Soil and Sediment - Biosolids/Sewage Sludge / digestate "]
[null,"Dust"]
[null,"Biota (animals) - Fish (organ / tissue)"]
[null,"Biota (animals) - Invertebrates"]
[null,"Biota (animals)- Cetacean (organ / tissue)"]
[null,"Biota (animals)- Land Mammals"]
[null,"Biota (animals) - Birds "]
[null,"Biota (animals)- Eggs"]
[null,"Biota (animals)- Milk"]
[null,"Biota (animals)- Blood / serum "]
[null,"Biota (plants and algae)  - Algae"]
[null,"Biota (plants and algae)  - Seaweed"]
[null,"Biota (plants and algae)  - Food (crops)"]
[null,"Biota (plants and algae)  - Whole Plant"]
[null,"Biota (plants and algae)  - Plant parts"]
[null,"Human  - Whole Blood"]
[null,"Human  - Serum  "]
[null,"Human  - Plasma"]
[null,"Human  - Urine"]
[null,"Human  - Breastmilk"]
[null,"Air and gasses - Indoor Air "]
[null,"Air and gasses - Outdoor air"]
[null,"Air and gasses - Flue gas  / stack emission "]
[null,"Air and gasses - Biogas "]
[null,"Filter Paper / Cartridges / Passive discs  "]
[null,"Products and Consumables - Paints and Varnishes "]
[null,"Products and Consumables - Cosmetics"]
[null,"Products and Consumables - Paper and cardboard"]
[null,"Products and Consumables - Fabric / carpet"]
[null,"Products and Consumables - Food Packaging (Paper)"]
[null,"Products and Consumables - Food Packaging (other)"]
[null,"Products and Consumables - Rubber and plastic"]
[null,"Other"]